3014MSC - Molecular Biology Methods Report Writing - Biology Assignment Help

Assignment Task

Introduction.

The cornerstone of scientific communication is the research paper, where the authors= outline their research results in a way so that any group should be able to replicate the work and scrutinise that way the experiments were undertaken and interpreted. The choice of methods is critical to the outcomes. Many research articles are limited by the methods they use. The purpose of this task is to strengthen your understanding of current molecular biology methods by comparing them to older approaches and their advantages in relation to previous molecular biology methods.

This paper used a technique called Differential Display-PCR (DD-PCR) to clone a novel interferon regulated gene and then sequence it and confirmed its regulation by interferon (IFN) using Northern blotting techniques.
After reviewing the paper you must outline how you would go about isolating the gene for human ISG60, clone it, and express it for human use using modern molecular biology techniques.

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