Amplification Of Partial Length CDNAS - Geldoc Imaging System Science Report Assessment Answer

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Geldoc Imaging System Science Report Assessment Answer

TASK:  Step 1: Isolation of RNA from rice tissues Each group will be given an Eppendorf tube which contains 30?L of RNA solution that has already been extracted for you. Keep your RNA on ice! RNA is from either the roots, leaves, shoots or seeds of the japonica rice cultivar Nipponbare, which has a fully sequenced genome. Step 2. PCR You will assay for the activity of two genes: a gene encoding a member of the serpin family of proteins known as “LRS” and a control “housekeeping” gene, actin. The “LRS” gene ( referred to as ‘serpin’ from here on in) has locus number Os03g41419 — this means “Oryza sativa (rice), chromosome 3 position 41419”. The actin gene has locus number Os03g50890. You will use PCR primers that allow amplification of a partial-length cDNA for each of the two genes. The PCR primers (designed and tested previously) for the two genes have the following sequences:

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