BB3802 - Flow Cytometry Principles and Applications in Research Assignment

Assignment Task

The coursework should address the following sections

• Describe the principles of flow cytometry and its purpose and compare these with other technologies such as immunohistochemistry (that were covered at Level-2). Describe the various applications of flow cytometry in basic research and medicine (with one example in detail).
• Analyse and explain the standard flow cytometer result provided below

• Analyse the flow cytometry data below. Describe what the cell subsets could be in each circled population. Discuss the interactions that might be expected among macrophages, T cells and B cells in the situation of a viral infection.

Discuss the following questions:

a) What fluorescent molecules have been used for each antibody in this experiment?

b) Why is mouse IgG1 needed in this experiment?

c) What could the cell populations be for R1 (top left), R2 (top right), M1 for IgG1 (bottom left), and M1 for IL-4 (bottom right)?

d) What conclusion can you make from this flow cytometry readout?

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