Bio- Chemistry - SPECTROPHOTOMETRY FOR THE QUALITATIVE AND QUANTITATIVE MEASUREMENT OF BIOCHEMICAL COMPOUNDS

Question : Apply the principles of spectrophotometry to the qualitative and quantitative measurement of biochemical compounds.   method :    The measurement of inorganic phosphate is one of the most common determinations carried out in a biochemistry laboratory and is a useful exercise for checking the validity of Beer's Law. Inorganic phosphate reacts with ammonium molybdate in an acid solution to form phosphomolybdic acid. When a reducing agent such as 1amino2naphthol4sulphonic acid is added to the molybdic compound bound to phosphate, it reduces the molybdenum atoms (VI to V), to give a blue coloured complex, but does not affect the unreacted excess molybdic acid, which remains colourless.