BIO3204 - Molecular Diagnostics 2 Practical Assignment

Assignment Task

Activity 1

Results

Insert two figures as indicated at res school and PowerPoint and on the task sheet.

Ensure that it has the formula of the standard curve along with the results highlighting the size of any potential bands observed on the electrophoresis.

Write 1-3 sentences describing the results obtained in the figures and the size of the target sequence.

Answers to Questions

a) Is Paige Turner is HIV positive?

b) What is the size of the nucleic acid markers used to test for the target sequence and what could be the target sequence for this assay based on the size of nucleic acid markers?

c) Is the target sequence for this assay is appropriate to be used as a future target sequence and why?

Activity 2

Results

Insert two figures as indicated at res school and PowerPoint and on the task sheet.

Ensure that it has the formula of the standard curve along with the results highlighting the size of any potential bands observed on the electrophoresis.

Write 1-3 sentences describing the results obtained in the figures and the size of the target sequence.

Answers to Questions

a) Is there is enough evidence to confirm a HIV diagnosis for these patients based on the results provided including a brief justification as to why or why not?

b) Why western blotting is not used routinely in clinical laboratories and what could be an alternative molecular diagnostic technique utilized to confirm HIV infection status

c) Extension questions

1. The patient in question here is patient C. Does this result confirm that she is positive for HIV-1, based on Australian and/or World Health Organisation Guidelines? Please explain your answer.
2. What if the patient in question was patient B. Would this confirm you that she is positive for HIV-1, based on Australian and/or World Health Organisation Guidelines? Please explain your answer.
3. Presuming the Western blot results confirmed the serological findings, what technical reason may explain why a viral load or <75>
4. Presuming the Western blot results confirmed the serological findings, what else, other than a technical reason, may explain why our patient has repeated positive serological testing and an extremely low or unquantifiable HIV RNA-1 viral load?

Activity 3

Part A of Activity 3:

Insert a brief overview including points from the task sheet into a fluent piece of writing

Part B: Results

Very brief as it should only contain 1 Figure and 1-2 sentences.

Part C of Activity 3

Activity 4

Part A of Activity 3:

Insert a brief overview including points from the task sheet into a fluent piece of writing

Part B: Results

Very brief as it should only contain 1 Figure and 1-2 sentences.

Part C of Activity 4

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