BIOL 3762/8005: Module Protein Purification - Essay Writing Nursing Assignment Help

Assignment Task:

Module Protein Purification assessment Question;

1. Using NCBI website as we discussed in the workshop for Module 1, find the sequence for Arabidopsis Hexokinase.  Design a purification procedure using Ion-exchange Chromatography, taking into account that you wish to purify it in its native form. Using bioinformatics determine its Molecular weight and discuss how you would confirm this proteins molecular weight and subunit composition experimentally.

Module Vesicular Traffic assessment questions

Please answer ONE of these questions using no more than one –two A4 page(s) per question (not including any diagrams or Tables) you may wish to use.

You can use other resources, but please reference this of you do using the Harvard system.

Each student needs to submit their own work and check with Turnitin.

1. A pharmaceutical company has decided to design a new drug that will treat a dis-order which is due to continuous nerve action. Using your knowledge of endocytosis, vesicle transport and specific targeting, design and explain the mechanism of a drug which will stop neurotransmitter release in pre-synaptic neurons. (Hint:explain the cellular processes involved as part of your answer)

OR

2. Using your knowledge of how proteins are imported into the Endoplasmic reticulum (ER), the processing of proteins in the ER and Golgi compartments and how vesicles are formed and delivered to specific destinations, discuss how lysosomal proteins are delivered to the lysosome. In your discussion, indicate a defect in this process that presents as a Lysosomal Storage Disease in affected patients.

Module  Protein Structure Determination assessment Question;

1. G-coupled protein receptors are an important class of membrane proteins which are important drug targets.  Describe the approach and technique(s) you would take,  including your rationale for your choice of technique(s), to determine the molecular structure of a particular G-coupled protein receptor that you have identified and cloned.

Module Protein: Protein Interactions assessment 

1. ATP synthase is a well conserved multimeric protein.  All organisms studied to date contain homologs of 8 different subunits which are essential for the complexes function.  However, you have just completed sequencing the genome of a novel organism, which despite having a functional ATP synthase, it only has genes which are homologous to 5 of the 8 essential subunits. On the basis of these findings you hypothesise that the ATP synthase of the novel organism contains novel subunits which fulfil the same functional role as the missing essential subunits.  Describe, a series of experiments you could perform to identify the novel subunits and the genes that code for them. Explain your rationale for the selection of technique(s) you have used.

Module  Mass spec from 2019 was:

Read the paper provided in Module 6 titled ‘Mass spectrometry based proteomics: existing capabilities and future directions’. Discuss benefits and shortcomings of two different Mass Spectrometry (MS) methods discussed in the paper. Give an example of a hypothetical experiment where the first MS method you chose to write about could be applied, and why it would be more appropriate to apply that method. Also give an example of an experiment where the second MS method you chose could be applied, and why it is more appropriate.

This question is appropriate for revision,  but would be more work than would be expected in online exam question as you would need to read the paper. The paper is already on FLO in the mass spec module.

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