BIOL2230 - Action Potential Lab Report Writing - Nursing Assignment Help

Assignment Task

 Action potentials from sensory nerves in the isolated cricket leg

Q-In Excel (or similar), plot a labelled scatterplot graph of mean RMS volume difference (the proxy for your neural response) against distance (in cm) over the range of distances at which you provided a puff stimulus using the syringe.  You may connect your points with a line if you choose to do so, but do not fit a curve to your data.  Decide which is your X axis and which is your Y axis.  Label your axes remembering to give units.  Give your graph an appropriate title. Copy your graph (directly or using the PrtScn function) and paste it here (as an image).

Q-Interpret the results you obtained in terms of stimulus strength, rate coding and the relative refractory period. Did your data show the relationship you expected? If not, why might that have been? Illustrate your answer with images of the recordings taken from your preparation 

Q-In this experimental set-up, we specified a sampling rate (project rate) of 44100 Hz.  What would be the consequences for our electrophysiological measurements of spiking activity if we had used a much slower sampling rate and why?

Q-Consider the traces you recorded from the cricket leg. Give some different reasons why the spikes you observed in the recordings have different amplitudes, especially when the strength of the stimulus changed, even though an action potential is supposed to have a more-or-less constant amplitude as it travels along an axon. 

Q-Reflect on the ethics of using crickets in a practical class. Is it justifiable? 

Q-Why does a Faraday cage around your preparation reduce noise in your recordings?

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