BIOL8005: The Reaction of AOX with DTT - Medical Sciences Assignment Help

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Assignment Task:

Please answer the following questions in half a page of text (Times Roman 12 point, single spacing). This does not include any diagrams you wish to use.

If you use information or diagrams from another source, you must reference them. 

 

  1. Please explain how the images on your blot were generated. What forms of the AOX protein are present in each sample? (5 marks). You can use your own blot or the one on FLO which Aparna and Vanessa made.

  2. Explain the reaction of AOX  with DTT and which amino acid (residue number) is involved. You should use  a reaction diagram. (10 marks)

  3. Explain the reaction of AOX  with Diamide and which amino acid (residue number) is involved. You should use a reaction diagram. (10 marks)

  4. If you wanted to have a form of AOX that was always active in the cells of plants, how could you generate this? ( 10 marks)

 

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