Highlights
INTRODUCTION
First part: Biosurfactants (what they are, why they are important, which microbes have the ability to produce biosurfactants)
Second part: Bacillus subtilis and biosurfactants (to explain why Bacillus subtilis is commonly used in fermentation, and why it is the good producer of biosurfactants) Third part: a summary. * Please pay more attention to the connection between paragraphs, and sentences.
MATERIALS AND METHODS
First part: microorganisms and culture (which microorganism you are using, where it is from, the medium composition, and culture conditions) Second part: scale-up fermentation (the culture conditions used in fermenter) Third part: analytical methods (how to measure OD600 of your samples, how to measure the surface tension) Fourth part: statistical analysis (optional)
RESULTS AND DISCUSSION
Present your data and discuss the results First part: the optimal temperature for biosurfactant production in Bacillus subtilis Second part: Scale-up fermentation in a bioreactor For discussion, you should discuss what your results mean, how it compares with other researcher's results, what understanding it might lead to, the weakness of your work, and potential future research plan.
CONCLUSION
Should not be more than one paragraph and should not have more than words
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