Cell Counting Prac Report - Science Assignment Help

Assignment Task

CELL COUNTING PRAC REPORT
INTRODUCTION
-Cover and expand on main terms at a minimum you should cover
-Why count cells
-What are the different methods (manual, semi automatic, automatic) how do they work and give examples (ie for manual define and explain how a haemocytometer works and how to use it)
-What are advantages and disadvantages and or different context in which you might use each method
-What is trypan, how do you use it

MATERIALS AND METHODS
- Please cite the manual in the correct APA/HARVARD format and note the changes (we changed a few things for example no Part D)

DISCUSSION (these are some points)
- Start with an overall observation with trends ie were the manual and automatic counts overall similar or not
- Go into more detail by looking at the pairs with outliers- what may have caused these differences? (look at steps in protocol to identify where human error can be introduced, look at/google the manual for the countess and how the algorithm works-the trouble shooting section can be quite handy)
- The pairs were an internal control-remember that the pairs were from the same suspension of cells so they should have very similar results, the variable is the technician
- What does the research say about the two different methods accuracy and does ourdata show this too? (ie are both methods accurate? Is one more accurate than the other? Does it depend on context?)

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