CSA742: how cell culture can help to understand drug-induced adverse - Report Writing

Internal Code: MAS 4660

Report Writing

Question 1: Discuss how cell culture can help to understand drug-induced adverse effects observed in a patient. Question 2: How can cellular toxicity be monitored in vitro. Use at least 5 different and independent parameters (do not list assays!) that could help you to get a  etailed understanding of the type of toxicity that is present. Question 3: Discuss the suitability of the WST-1 assay to determine cellular toxicity and list advantages and disadvantages. Also discuss potential artefacts that could affect the results obtained from this assay. Question 4: Discuss the suitability to measure cellular ATP content to determine cellular toxicity and list requirements, advantages and disadvantages. Also  iscuss potential artefacts that could affect the results obtained from this assay. Question 5: Describe the toxicity (what does it mean) in the following scenario and describe the resulting danger. After exposure of live cells to a novel drug the following is measured in cell culture: no changes in trypan blue assay, increased gammaH2AX signal, no changes in WST-1 assay, no changes in ATP  essay, no changes in apoptosis, no changes to protein content/well. Question 6: Give one example how a viability assay can be used to measure something entirely unrelated. Support your argument by a detailed reaction scheme. Question 7: Describe why many viability assays are standardized on protein content. What is the advantage?