Design and Construct a Suitable Vector for Transforming - Management Assignment Help

Assignment Task :

Background brief on global challenges: The world is facing a carbon and freshwater constrained economy, which is exacerbated by finite resources (fossil fuel & land). The global population will be exceeding 9 billion people by 2050, which will place additional demand on resources with increases of 70, 50, and 30% being predicted for food, fuel, and water, respectively by 2050. Agricultural production to meet the predicted 70% increase is challenged by scarce freshwater availability and greenhouse gas emission induced climatic instability, as well as diminishing arable land. Algae, including the photosynthetic cyanobacteria to which Synechococcus elongatus belongs, have been heralded to be the potential saviours, as the fix atmospheric carbon dioxide (a greenhouse gas), converting it to organic carbon compounds, such as protein, carbohydrates and lipids, and they can be grown on non-arable land in non-potable water.

Your assignment aims and tasks:
Aims of your experimental design:
To produce a temperature- and salinity-tolerant S. elongatus through transformation with the ectoine gene cassette from the extremophile H. elongata

Tasks:
1) Design and construct a suitable vector for transforming S. elongatus with the ectABC gene cassette from H. elongata
2) To produce a detailed experimental design flow chart that outlines succinctly all steps required.
3) Produce separate detailed specific experimental designs for all required processes.
4) Based on the above, write a materials and methods section, referring back to the produced diagrams as figures.

Specific instructions:
The organism to be transformed is Synechococcus elongatus PCC 7942. Ectoine gene sequence to be used:
For your transformation experiment use the ectABC gene sequence from Halomonas elongata DSM 3043 (Genbank accession no: AJ011103).
For this assignment, proceed with developing your experimental design for ectoine transformation of Synechococcus elongatus by following the steps that were worked out in a series of preparative workshops using transformation of CHO cells with the human interferon gamma gene.

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