Highlights
Questions
1. There was a dog at the scene. However, the ABAcard Hematrace test was not used on item 1. Explain why this test wasn’t used for this item and not generally used for most crime scenes.
2. Would item 2 be submitted as a differential extraction? Explain.
3. Provide two different explanations for how the underwear (item 4) was AP and P30 positive while the microscope examination was negative.
4. What body fluid tests, if any, would you request for item 7?
Questions
1. Based on the quantification results, do items 1 or 2 give any indication that they might be degraded? Explain.
2. Based on the quantification results, do any of the samples appear to be inhibited? Explain.
3. For item 5, you are allowed to request one amplification. Would you select an autosomal STR or a Y-STR amplification? Explain.
4. Referring to the quantification results for item 7 above, describe how you would set up a 1.2 ng Y-STR amplification reaction. You have 50 µl of the DNA sample and your lab uses 10 µl of template DNA in a 20 µl PCR reaction
Note: The minimum volume available to pipet is 1 µl.
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