Heme Thiolate Monooxygenases - Swiss Prot Protein Database - Chemistry Assignment Help

Assignment Task :

Q1 Hexokinase is an important enzyme which is part of the glycolytic pathway.
There are several forms of the enzyme, one of which is highly expressed in cancer cells.
a) Use the NCBI or EBI portals to retrieve one file for each of the several different forms of hexokinase found in humans. Each file should contain the complete mRNA sequence (it is not necessary to print the sequence out).

Q2 Cytochrome P450s are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
In this question you will explore the relationship of the Cytochrome P450 1A1  sequence for a number of different species.
 

(i) Using UniProt locate the sequence for the full-length human sequence. Then run a BLAST search for this sequence at the NCBI site (not from UniProt) against the Swiss-Prot protein database and identify 7 other different species of the protein with close similarity to the human sequence (make sure they are full length sequences).

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