HSE110 : Muscle Biology for Exercise Laboratory Report Submission Template Laboratory Report Intensity and Muscle Glycogen Utilisation - Medical Science Assignment Help

Assignment Task:

Task:

1. Background

1.1 What are the main metabolic fuels (including the different forms of each fuel) that can be used to power skeletal muscle contraction during exercise? (~50 words)
In paragraph format and using the suggested word count as a guide (try to write as succinctly as possible), answer the above question here (delete this red text prior to submission).
In paragraph format and using the suggested word count as a guide (try to write as succinctly as possible), answer the above question here (delete this red text prior to submission).

1.2 What is the influence of relative exercise intensity on the metabolic fuels used to power skeletal muscle contraction during exercise? Cite 1-2 studies to support your argument (see example readings list provided in the Topic 1 data analysis manual). (~100 words)
In paragraph format and using the suggested word count as a guide (try to write as succinctly as possible), answer the above question here (delete this red text prior to submission).
In paragraph format and using the suggested word count as a guide (try to write as succinctly as possible), answer the above question here (delete this red text prior to submission).

1.3 Considering both your answer to question 1.2 and the research question for the experiment (see the Topic 1 workshop manual), what was your aim and hypothesis for this experiment? (~50 words)
In paragraph format and using the suggested word count as a guide (try to write as succinctly as possible), answer the above question here (delete this red text prior to submission).
In paragraph format and using the suggested word count as a guide (try to write as succinctly as possible), answer the above question here (delete this red text prior to submission).


Methods (this has been completed for you)
To investigate the effects of exercise intensity on muscle glycogen utilisation, a randomised control trial was utilised. Eight participants were randomised into an acute bout of cycling at either a moderate or high intensity. Participants were recreationally active but not trained, and included four males and females between 18-30 years.
Participants arrived to Deakin University in the morning after an overnight fast. Anthropometric data were collected including participants’ height, age and body mass. A resting muscle biopsy sample was taken from the vastus lateralis using the Begrstom technique, and blood lactate was quantified using a venous blood sample from a finger prick. The exercise session consisted of 60 minutes on a cycling ergometer at 50% VO?peak for moderate intensity, and 70% VO2peak for high intensity. Blood lactate samples were collected every 10 minutes throughout the exercise protocol. Immediately after the exercise, another muscle biopsy was obtained from the vastus lateralis. Skeletal muscle samples were then snap frozen in liquid nitrogen and stored at -80°C until analysis.
Skeletal muscle samples were freeze dried and powdered for analysis of glycogen content using a glucose oxidase assay. Samples were prepared in triplicate with a standard curve of 0 - 10nM glucose. Hydrochloric acid was added to the sample and incubated at 95-100°C for two hours to hydolyse the glycogen into glucose molecules. Potassium hydroxide was added to neutralise the solution, then glucose oxidase, glucose peroxidase and 4-Aminoantipyrine were added to the solution. Samples were incubated at room temperature for 15 minutes, then read on a spectrophotometer at 490nm.


2.1 Summarise, in table format, the average participant age, height and body mass the Topic 1 research experiment. Include an appropriate table description above the table (not included in word count).
 

REMOVE TEXT AND INSERT TABLE HERE
2.2 Based on the results of the experiment provided in the Excel data sheet, generate an appropriate graph demonstrating the blood lactate responses to moderate- (50% VO2peak) and high-intensity (70% VO2peak) exercise. Include an appropriate figure description below the figure (not included in word count).
 

REMOVE TEXT AND INSERT GRAPH HERE
2.3 Based on the results of the experiment provided in the Excel data sheet, generate an appropriate graph demonstrating muscle glycogen use with moderate- (50% VO2peak) and high-intensity (70% VO2peak) exercise. Include an appropriate figure description below the figure (not included in word count).
 

REMOVE TEXT AND INSERT GRAPH HERE

3. Discussion and interpretation
(8 marks)
3.1 What were the main blood lactate and glycogen usage findings of the experiment? Are the findings of this experiment consistent with your hypothesis as stated in question 1.2? (~150 words)
In paragraph format and using the suggested word count as a guide (try to write as succinctly as possible), answer the above question here (delete this red text prior to submission).
In paragraph format and using the suggested word count as a guide (try to write as succinctly as possible), answer the above question here (delete this red text prior to submission).

3.2 Are these findings consistent with previous research related to this research question? Why/why not? Cite 2-3 studies to support your argument (see example readings list provided in the Topic 1 data analysis manual). (~200 words)
In paragraph format and using the suggested word count as a guide (try to write as succinctly as possible), answer the above question here (delete this red text prior to submission).
In paragraph format and using the suggested word count as a guide (try to write as succinctly as possible), answer the above question here (delete this red text prior to submission).

3.3 Conclude your discussion by summarising the main findings and the associated implications for real-world practice: (~150 words)
In paragraph format and using the suggested word count as a guide (try to write as succinctly as possible), answer the above question here (delete this red text prior to submission).
In paragraph format and using the suggested word count as a guide (try to write as succinctly as possible), answer the above question here (delete this red text prior to submission).
 

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