Human gene Mapping Laboratory - Genetics - Nursing Assignment Help

Assignment Task:

HUMAN GENE MAPPING LABORATORY 

For this lab, you will be mapping a gene that is associated with an inherited form of a human disease. This is a multi-step process: 1) determine the mode of inheritance for the disease in the family; 2) analyze pedigrees to determine which chromosome the gene is on; and 3) map the position of the disease gene relative to a couple of markers with know map positions using LOD analysis. 

You will be assigned one of three genes (A, B, or C) for your study. Each is associated with a PowerPoint file posted on the course Canvas site. 

When you open your file, the first slide will have a pedigree showing a large family that exhibits the disease. Based on your analysis of this pedigree, determine the mode of inheritance for the disease allele in the gene in this family. 

Underneath the pedigree, you will find a set of chromosomes maps showing a small portion of 5 different chromosomes. Chromosome markers (representing RFLP probes) are shown on the maps with their known map positions on that chromosome. 

For many of you, it will be necessary to do some more work to determine which chromosome the gene is on. If that is the case, the next slide will show the results of an RFLP experiment for 4 different markers each from 1 of the 4 remaining candidate chromosomes. For each pedigree, fill in the results of your analysis (# recombinants & # parentals) on your lab report. Determine which chromosome the gene is on by asking which marker is linked to the disease gene. 

The remaining slides shows the results of RFLP experiments for two different markers on the chromosome. For each marker, note the map position on your lab report and fill in the results of your analysis for each pedigree in the table. In addition to # recombinants and # parentals, there is a column labelled NA. In some cases, it is not possible to develop a hypothesis for linkage between the disease allele and the (+) or (-) RFLP alleles or it is not possible to unambiguously follow the RFLP allele into the third generation of the pedigree. In those instances, you should just put an "X" in the NA column. 

Once you have completed your analysis of the pedigrees, total up the # recombinants and # parental for each marker. Based on that data, estimate the map distance between the marker and the disease gene then calculate the LOD score for that estimate. 

Once you have estimated the map distances from the two markers, propose a final map position for the disease gene.

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