Measurement of Plasma Protein from Blood Samples - Medical Science Assignment Help

Assignment Task

Question You have constructed the following standard curve for measurement of plasma protein from blood samples below. Nominate 3 quality control concentrations, providing a brief justification as to the range of values chosen.

You take 100 l of blood plasma to analyse protein levels from a patient. You dilute this 1 in 10 and then measure the absorbance. The Absorbance value = 0.6. Calculate the approximate plasma protein concentration of this patient in grams per litre (g/L).

Question Pharmacologists have discovered a drug (X) which influences central nervous system function. In characterising drug X, mice dosing studies (n= 24 per group) revealed the following data on sleeping time when drug X was administered together with known centrally active drugs, diazepam (diaz) or ethanol (EtoH), all at pharmacologically active doses. The drug (X) has no effect on sleeping time when injected into rats alone (i.e. equivalent to saline (sal) injection, sleeping time = 0). Given the following data:

Describe the types of interactions that Drug X has with i) ethanol and ii) diazepam, explaining the scientific rationale for your answer. Can you determine the pharmacodynamic nature of Drug X and what possible clinical uses might it have?

Question. A piece of Guinea Pig Ileum is suspended in an organ bath attached to a force transducer and the contractile response to various compounds tested. You have constructed a series of doseresponse data below, based on your tissue response to histamine, alone and in the presence of 3 concentrations of the antagonist ketanserin.

a) Complete the following table, given that the nonlinear regression curve falls on the points tabled and the dose ratio is equivalent to the EC50 [agonist] in the presence of [antagonist] ÷ EC50 [agonist] alone.

b) Graph the Schild Plot on the next page, with appropriately labelled axes. From the graph, state the (approximate) dissociation constant (KB) and pA2 for ketanserin. What does this pA2 value represent? [4 marks] c) If another drug has a higher pA2 value than ketanserin, what does this tell you about the drug’s pharmacodynamic properties in comparison?

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