Medical Biochemistry Laboratory Report Writing - Medical Science Assignment Help

Assignment Task

Task

Introduction:

Why you did it”.

The introduction section presents the relevant background to the experiment. You should write for your audience (your peers) - you can assume that the reader has a sound basic scientific knowledge. Thus, you don't need to explain all aspects - give just enough so that reader can understand why you did the experiment. Use in-text referencing to refer to your sources and provide links for a reader that might require more background. DO NOT review the whole topic (that's what review articles are for!). Indeed, your introduction should be as short as possible.

Results:

“What you found”

This section should describe your results but not interpret them (save that for the discussion).  The results may be represented graphically, OR tabulated OR in words (don't present the same data in more than one style). However, it is not enough to just give the data - you must also describe the changes that you observed, referring to figures and/or tables.  Ensure tables and/or figures have legends that describe the relationship illustrated. The results should be described in the body of the text, not just in the figure or table legend.

Discussion:

First should address the aim of the study.  Outline any implications of your results and any problems you encountered. Answer questions that were in the experiment & use references & your results to support your answers. In text referencing should be included.

Questions:

1. Which fraction contained the highest amount of albumin? Lysozyme?

2. What evidence is there that the ion exchange column separated the two proteins (refer to your graph)? 

3. What information have you gained about the overall charge characteristics of lysozyme and albumin? 

4. Explain, at the molecular level, why a high ionic strength buffer can elute the protein analyte of interest.

5. What results might you have expected if you used a cation-exchange resin? 

6. Provide one (1) other practical application (used in research or industry) for IEC. 

7. Predict the order of elution of the following amino acids (number 1st  to 5th ) from an anion-exchange column at physiological pH:-    

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