MIC11100 - Molecular Pathogenesis of Microbial Infection

Assignment Task

Question

1. Questions 1A and 1B refer to the paper: Reis et al., (2010) LapB, a Novel Listeria monocytogenes LPXTG Surface Adhesin, Required for Entry into Eukaryotic Cells and Virulence

A - A Western blot showing levels of expression of known L. monocytogenes proteins involved in invasion. Describe in turn how each of these proteins contributes to L. monocytogenes virulence.

B - In your own words, critically discuss what Fig 7A tells us about the contribution of LapB to virulence.

2. This question relates to the paper: Deng et al., Effects of Mycobacterium tuberculosis Rv1096 on mycobacterial cell division and modulation on macrophages.

3. This question relates to the paper: Hung, CC, Haines, L & Alter, C The Flagellar Regulator fliT Represses Salmonella Pathogenicity Island 1 through flhDC and fliZ, PLoS ONE 7(3): e34220.

4. This question is based on the paper: Gonzalez CD, Ledo C, Giai C, Garófalo A, Gómez MI (2015) The Sbi Protein Contributes to Staphylococcus aureus Inflammatory Response during Systemic Infection. PLoS ONE 10(6): e0131879.

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