Micro-Computed Tomography (Micro-CT) Scan - Raman Spectroscopy - Research Assignment Help

Assignment Task:

Task:

Scanning Electron Microscopy (SEM)

Scanning electron microscopy works on the following principle: a high energy electron beam is sent to the sample and scan the surface. Depending on the constituting elements which make up the sample, the electron beam gets reflected (into secondary signals) accordingly and is sent to a detector, which processes the signals and produces a computer image. The samples' electrical conductivity defines the electron beam energy to be used for testing; a highly conductive sample requires higher energy bombardment for analysis while materials of lower conductivity can only be tested at lower electron volts (keV), otherwise the samples can suffer from charge accumulation on the surface, a phenomena known as "charging".

Raman Spectroscopy

When a molecule absorbs energy, either one of the scatterings occur: Rayleigh or Raman scattering. Rayleigh (elastic) scattering takes place when a molecule absorbs and emits radiation with the same energy while in Raman (inelastic) scattering, the molecule either emits a higher or a lower energy wave than the initial photonic excitation. For measurements done in this work, Witec Alpha 300 RAS (Figure 15) was used with the green light laser source of 532 nm wavelength. The monochromatic light hits the sample which is focused in the z -direction for maximum interaction on a molecular level.

Micro-Computed Tomography (Micro-CT) Scan

Micro-computed tomography (Micro-CT) scanning relies on transmitting x-rays through a 3D sample to get images on a slice-by-slice method. X-rays are generated by directing of electrons produced in a cathode (tungsten, copper, etc.), similar to the XRD principle. The electron bombardment on the target rejects x-rays which are sent to the sample for imaging. Some x-rays are absorbed by the sample while others travel through.

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