Molecular Mechanisms and Vitro Osteoclast Genesis and Resorptive Activity - Medical Science Assignment Help

Assignment Task

The cells examined in our primary article experiment were isolated monocyte and macrophage  populations, these were the RAW264.7 mouse cell line and hematopoietic cells which were cultured  with macrophage colony stimulating factor to induce differentiation into monocyte populations. The  experiment focused on three main aspects of testing IL-10 efficacy. 

- In vitro osteoclastogenesis and resorptive activity 

- Molecular mechanisms 

- And IL-10 Efficacy

Cells were cultured with 50ng/mL of RANKL with varying concentrations of IL-10- nil IL-10 was used as  a negative control. The reagents were refreshed at 3 days and then after 6 days subjected to tartrate resistant acid phosphatase staining. Positive TRAP staining indicates differentiation into osteoclast  cells. The activity of these cells were measured by plating onto dentine slice assays in the presence of  the same culture medium. Toluidine blue staining of the slices allows for microscopic observation of  osteoclast resorptive activity through pit formation.

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