Msc Molecular Biology and Biotechnology Coursework - Biology Assignment Help

Assignment Task

Task
a. Detail how protein expression will be induced in your chosen expression system
b. Set out an experimental scheme to lyse and collect cytosolic fraction containing your target protein. Pay careful attention to the components of your lysis buffer
c. Using the information given about the protein and the simulation software detail the protein purification protocol that you will use to isolate the Q-protein. You will require a yield > 80% and >30 m

d. Once isolated you will need to confirm that the protein has retained its biological activity. To do this perform at BLAST search using the either the DNA sequence or transcribed amino acid sequence to determine the class of protein and then identify a suitable enzyme assay. 

1- What pH will the purification be performed at?
2- What is the charge on the protein at this pH?

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