Mystery Recombinant Protein - Protein is Known as VCH_cass14 - Nursing Assignment Help

Assignment Task:

Your protein is known as VCH_cass14. 

The VCH_cass14 gene was cloned for over-expression in the bacterial host Escherichia coli (BL21 (DE3) pLysS). The protein sequence was engineered with additional six-histidine residues at its N-terminus. The protein was discovered within the mobile gene array (at cassette 14) of a marine-dwelling species of Vibrio cholera, and remains of unknown function. 

The Protein Structure Group at Macquarie University solved the x-ray crystal structure of the protein, and you can find details in the Protein Data Bank entry (ID 3IMO). With this, including the extra His residues, you will be able to calculate precise molecular weight of VCH_cass14 using the ExPASY ProtParam tool. 

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