Proposed Cytometer and Spectrum Viewer - Medical Science Assignment Help

Assignment Task

Cell count

1. Gently mix the tube of FBMC by inverting several times
2. Pipette 20uL of cells into a small tube or an unused with of the 96-wll plate
3. Pipette 180uL of Trypan blue counting reagent into the same well. Mix by pipetting
4. Using a fresh pipette, transfer 20uL of the celUTrypan blue mixture into the
5. the equation X x l0^4 (ceus/ml) x 5 (ircounring 5 squares) x dilution factor

Trvpsin:

Proposed Analvsis (Briefly describe the proposed data analvsis pathwav): To examine the effect of cell surface exposure of trypsin, flow cytometry will be used to analyse the cell samples and to determine the cell population and surface expression FlowJo software will also be used. Depending on where the trypsin enzyme cleaves within the polypeptide chain, the antigen expression will be modified on the leukocyte cells at the trypsin cleaving site.
 

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