Report - Lactate - DNA - Report Writing Assignment Help

Internal Code : I_AH_CGAC_AFH

Report Writing Assignment Help

TASK Develop an assay to measure lactate in cell culture media (not inside cells!) using the information we learned in the tutorial and workshops. For this purpose: Provide a complete reaction diagram that explains YOUR assay Describe the specific enzyme you intend to use (source, unit definition, optimal reaction parameters) Give all details AND explanation for the assay conditions (i.e. temperature, incubation times, reaction buffer composition, assay volumes) Where you obtain the chemicals and reagents necessary for the assay (incl. order number, do NOT use components of a commercial kit! This is cheating) Provide a step-by-step protocol, how to do the assay and what positive and negative controls to include