SCI302: Analytical Sciences - Gas Chromatography - Science Assignment Help

Assignment Task :

QUESTION 1:  Data Analysis 

The concentration of caffeine in softdrink was determined by HPLC with Internal Standard comparison. A series of caffeine working standards was prepared by diluting a 10.0 mg/mL stock by the following factors: 0.00 mL into 1.00 mL, 0.10 mL into 1.00 mL, 0.20 mL into 1.00 mL and 0.50 mL into 1.00 mL. The standard dilutions and softdrink sample (all 1.00 mL) were spiked with the same quantity of Internal Standard, then analysed by HPLC. The results are tabulated below. Determine the caffeine

QUESTION 2:  Gas Chromatography 

a) Describe how compounds travel through a GC capillary column. 

b) How can a GC be operated to effect rapid separation of a complex mixture of compounds of wide volatility range, (assume the compounds can pass through a GC)?

2.How does a Flame Ionisation Detector measure the quantity of eluting compounds in Gas Chromatography?

d) Why is Gas Chromatography unsuitable for analyzing mixtures of peptides?

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