Somatostatin Expressing Neurons in Cortical Networks Assignment

Assignment Task

Gene Mstg1 has a widespread expression pattern in the CNS during development. One of the phenotypes of mutant mice is reduced inhibition in the adult cerebral cortex caused by a reduction in Somatostatin (SST)-expressing cortical interneurons. Experiments were undertaken to determine whether the defect arises during development, whether it affects migration and which aspects of migration it affects. Explain what each of the panels A - D show, what experiments were performed in each case and what the results in each panel demonstrate. Summarise the findings from all the experiments and suggest possible functions for the MSTG1 protein.

Figure legend

A. Transgenic mouse lines used

B. Representative images from control and Mstg1 CKO cortices at E13.5 showing Sst [removed]blue signal). Quantification in control and cKO embryos is shown (counts presented as % of control). A scheme showing how cells were quantified is also shown. p<0>

C. Experimental design for assessing migration in D.

D. Images from control and Mstg1 cKO cells in culture and quantification of migration parameters. p<0> Abbreviations E2 E3 E4 exons cKO conditional knockout Sst somatostatin MZ marginal zone SVZ/IZ CP cortical plate subventricular zone/intermediate zone MGE medial ganglionic eminence distance.

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